THE 2-MINUTE RULE FOR NAD+ SUPPLEMENTS FOR ENERGY AND METABOLISM

Subsequent, we handled cells with distinctive concentrations of NAD+. When additional for 24 h at eighty µM in HeLa and 400 µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Extended Info Fig. 1k,l). Also, sixteen µM of NAD+ was sufficient to cut back fork pace by ~40% in HeLa cells without having impacting the overal